A low-cost protocol for the optical method of vulnerability curves to calculate P50

IF 2.4 3区生物学 Q2 PLANT SCIENCES Applications in Plant Sciences Pub Date : 2025-03-31 DOI:10.1002/aps3.70004

Georgina González-Rebeles, Miguel Ángel Alonso-Arevalo, Eulogio López, Rodrigo Méndez-Alonzo

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Abstract

Premise

The quantification of plant drought resistance, particularly embolism formation, within and across species, is critical for ecosystem management and agriculture. We developed a cost-effective protocol to measure the water potential at which 50% of hydraulic conductivity (P₅₀) is lost in stems, using affordable and accessible materials in comparison to the traditional optical method.

Methods and Results

Our protocol uses inexpensive USB microscopes, which are secured along with the plants to a pegboard base to avoid movement. A Python program automatized the image acquisition. This method was applied to quantify P₅₀ in an exotic species (Nicotiana glauca) and native species (Rhus integrifolia) of the Mediterranean vegetation in Baja California, Mexico.

Conclusions

The intra- and interspecific patterns of variation in stem P₅₀ of N. glauca and R. integrifolia were obtained using the low-cost optical method with widely available and affordable materials that can be easily replicated for other species.

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一种低成本的脆弱性曲线光学法计算P50的方案

植物抗旱性的量化，特别是物种内和物种间的栓塞形成，对生态系统管理和农业至关重要。与传统的光学方法相比，我们开发了一种具有成本效益的方案，使用价格合理且易于获取的材料来测量茎中50%的水力电导率（P50）损失时的水势。方法和结果我们使用廉价的USB显微镜，将其与植物一起固定在钉板底座上以避免移动。一个Python程序实现了图像采集的自动化。该方法应用于墨西哥下加利福尼亚州地中海植被的一种外来种（Nicotiana glauca）和本地种（Rhus integrfolia）的P50定量分析。结论采用低成本的光学方法，利用广泛、经济、可复制的材料，获得了青花云杉和整合叶云杉茎P50的种内和种间变异模式。

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来源期刊

Applications in Plant Sciences PLANT SCIENCES-

CiteScore

7.30

自引率

0.00%

发文量

审稿时长

12 weeks

期刊介绍： Applications in Plant Sciences (APPS) is a monthly, peer-reviewed, open access journal promoting the rapid dissemination of newly developed, innovative tools and protocols in all areas of the plant sciences, including genetics, structure, function, development, evolution, systematics, and ecology. Given the rapid progress today in technology and its application in the plant sciences, the goal of APPS is to foster communication within the plant science community to advance scientific research. APPS is a publication of the Botanical Society of America, originating in 2009 as the American Journal of Botany''s online-only section, AJB Primer Notes & Protocols in the Plant Sciences. APPS publishes the following types of articles: (1) Protocol Notes describe new methods and technological advancements; (2) Genomic Resources Articles characterize the development and demonstrate the usefulness of newly developed genomic resources, including transcriptomes; (3) Software Notes detail new software applications; (4) Application Articles illustrate the application of a new protocol, method, or software application within the context of a larger study; (5) Review Articles evaluate available techniques, methods, or protocols; (6) Primer Notes report novel genetic markers with evidence of wide applicability.