The expression of melanosomal matrix protein in the transdifferentiation of pigmented epithelial cells into lens cells

Cell differentiation Pub Date : 1988-03-01 DOI:10.1016/0045-6039(88)90045-0

Makoto Mochii, Takashi Takeuchi, Ryuji Kodama, Kiyokazu Agata, Goro Eguchi

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引用次数: 43

Abstract

A monoclonal antibody (MC/1) was constructed against melanosomes purified from the chicken pigmented epithelial cells (PECs) in order to characterize the differentiative phenotypes of PEC in the process of transdifferentiation into lens cells. Immunofluorescent studies revealed that MC/1 antibody specifically stains both retinal PECs in the eye and melanocytes in the skin, of chicken embryos. Immunoelectron microscopy showed that the antigen molecules are located on the peripheral region of the melanosomal matrix. A single protein band with an apparent molecular weight of 115000 was labelled by MC/1 in Western blotting. The 115 kDa polypeptide identified by MC/1 is considered to be a member of the melanosomal matrix proteins. The maintenance of specificity of pigment cell nature is followed in the system of transdifferentiation of PEC into lens in vitro, utilizing 115 kDa protein as a marker. In the dedifferentiated PECs, this protein was undetectable.

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色素上皮细胞向晶状体细胞转分化过程中黑素体基质蛋白的表达

为研究鸡色素上皮细胞(PECs)转分化为晶状体细胞过程中的分化表型，构建了针对PECs黑素体的单克隆抗体(MC/1)。免疫荧光研究表明，MC/1抗体特异性地染色了鸡胚胎眼睛中的视网膜PECs和皮肤中的黑色素细胞。免疫电镜显示抗原分子位于黑素体基质的外周区域。Western blotting用MC/1标记表观分子量为115000的单个蛋白条带。MC/1鉴定的115 kDa多肽被认为是黑素体基质蛋白的成员。以115 kDa蛋白为标记物，PEC转分化为晶状体的系统遵循色素细胞性质特异性的维持。在去分化的PECs中，这种蛋白是检测不到的。

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Cell differentiation

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