[Photometric control of the presence of potassium benzylpenicillin crystals after centrifugation].

Antibiotiki i meditsinskaia biotekhnologiia = Antibiotics and medical biotechnology Pub Date : 1987-10-01

E V Zosimov, E M Karbanov, E V Karpun, L Iu Sergienko, S A Nemchenko

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Abstract

A photometric (turbidimetric) method was proposed for automatic control of the content of potassium benzylpenicillin crystals after centrifugation. The optical properties of the technological media were studied. It was found that along with intensive absorption in the UV spectrum due to the presence of the penicillin and its degradation products the solutions absorbed the light of the visible spectrum (400-700 nm). Therefore, determination of the penicillin crystal concentration by light dispersion in the UV and visible spectra was possible only with a differential procedure. For the near IR region there were defined areas at 930-950, 1020-1080 and 1200-1260 nm without self-absorption of the solutions providing the use of the one-canal measurement scheme. Procedures for determining the presence of penicillin crystals by light dispersion were developed: differential procedures in the visible spectrum (490 nm) and the near IR region (940-980 nm). The use of market photometers for realization of the procedures was recommended.

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[离心后苄青霉素钾晶体存在的光度控制]。

提出了一种光度法(比浊法)自动控制离心后青霉素钾结晶含量的方法。研究了该工艺介质的光学性质。结果发现，由于青霉素及其降解产物的存在，溶液在紫外光谱中吸收强烈，吸收可见光谱(400-700 nm)的光。因此，通过紫外和可见光谱中的光色散来测定青霉素晶体浓度只有用微分法才能实现。对于近红外区域，在930-950,1020-1080和1200-1260 nm处有定义的区域，没有溶液的自吸收，提供了使用一管测量方案。开发了通过光色散测定青霉素晶体存在的程序:可见光谱(490 nm)和近红外区域(940-980 nm)的差分程序。建议使用市场光度计来实现这些程序。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Antibiotiki i meditsinskaia biotekhnologiia = Antibiotics and medical biotechnology

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