[Penicillin-selective electrode based on a cellulose membrane with immobilized enzyme].

A G Dobroliubov, Iu G Emel'ianov, D D Grinshpan, S B Itsygin, F N Kaputskiĭ
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Abstract

Modified cellulose films are recommended to be used as carriers in preparing enzymatic membranes for penicillin-selective electrodes. The results of laboratory testing of the enzymatic penicillin-selective electrode based on the cellulose membrane with immobilized penicillinase are presented. In principle it was shown possible to use the enzymatic electrode in determining penicillin concentration in solutions. Dependence of the electrode reading on the buffer capacity was revealed. Optimal characteristics of the enzymatic membranes and the life-expectancy of the enzymatic electrode were determined.

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基于固定化酶的纤维素膜的青霉素选择性电极。
建议使用改性纤维素薄膜作为载体制备用于青霉素选择性电极的酶膜。介绍了固定化青霉素酶的纤维素膜酶选择性电极的实验室试验结果。原则上,可以用酶促电极测定溶液中青霉素的浓度。揭示了电极读数对缓冲容量的依赖性。确定了酶膜的最佳特性和酶电极的预期寿命。
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