Development of cytotoxicity tests for assessment of the toxicity of water samples taken from the environment.

Molecular toxicology Pub Date : 1987-09-01
D J Benford, S Good
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Abstract

Different methods of measuring cytotoxicity have been investigated in order to establish a test system for assessment of the toxicity of water samples. Ideally this should be highly sensitive and rapid to perform. Four variations of the neutral red uptake test have been compared with the MTT test and ATP determination. Chinese hamster V79/4 cells were used as the test system with 10 model toxins. All tests gave essentially similar results, with linear regression analysis producing correlation coefficients in excess of .93. No single test was most sensitive to all 10 compounds. The neutral red uptake and MTT tests could be performed in a single working day (approximately 8 h) if test compound and cells were plated out simultaneously. These tests are preferable to ATP determination, which is a complex and lengthy procedure, requiring expensive reagents.

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开发细胞毒性试验,以评估从环境中提取的水样的毒性。
为了建立水样毒性评价的测试系统,研究了不同的细胞毒性测定方法。理想情况下,这应该是高度敏感和快速执行。中性红摄取试验的四种变化与MTT试验和ATP测定进行了比较。以中国仓鼠V79/4细胞作为10种模型毒素的试验系统。所有测试的结果基本相似,线性回归分析产生的相关系数超过0.93。没有一种测试对所有10种化合物都最敏感。如果被试化合物和细胞同时被镀,中性红摄取和MTT测试可以在一个工作日内完成(大约8小时)。这些测试优于ATP测定,后者是一个复杂而漫长的过程,需要昂贵的试剂。
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Xenobiotic regulation of glutathione S-transferase Ya gene expression. Review: gene amplification--a cellular response to genotoxic stress. Limitations of the fluorescent probe viability assay. Induction of a novel damage-specific DNA binding protein correlates with enhanced DNA repair in primate cells. Induction of a novel damage-specific DNA binding protein correlates with enhanced DNA repair in primate cells.
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