Immunohistochemical studies on the GABAergic system in the rat supraoptic nucleus using the PAP method with an application of electron microscopy.

K Iijima, K Ohtomo, R Kobayashi, N Kojima
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引用次数: 15

Abstract

GABAergic neuronal profiles in the supraoptic nucleus of the rat were immunohistochemically identified by using a purified GABA antibody with the peroxidase-antiperoxidase method. The localization of GABA-like immunoreactivity in nerve terminals on the neurosecretory neurons was examined electron microscopically. A few small GABAergic neurons were found inside the supraoptic nucleus while only a very few medium-sized ones were detected in the perinuclear zone. Intrinsic, non-GABAergic small neurons covered by GABAergic neuropil were also detected. The neuropil with GABAergic axo-somatic synapses evenly encompassed unlabeled neurosecretory perikarya throughout the supraoptic nucleus. The GABAergic system seems to inhibit both vasopressin and oxytocin cells. In this area, glia cells showed clear outlines of unlabeled somata around counter-stained nuclei. Blood capillaries in the supraoptic nucleus were only slightly covered with a GABAergic neuropil. Electron microscopic observations demonstrated the presence of GABAergic axo-somatic symmetrical and axo-dendritic asymmetrical synapses on the neurosecretory neurons. GABA-like immunoreactivity was localized on the membranes of microtubules and synaptic vesicles, on the external membranes of the mitochondria, and on the inner leaf of the presynaptic sites. Numerous pairs of non-immunoreactive synapses were arranged along these immunoreactive synapses.

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电镜下应用PAP法对大鼠视上核gaba能系统的免疫组化研究。
用纯化的GABA抗体和过氧化物酶-抗过氧化物酶法对大鼠视上核GABA能神经元谱进行免疫组织化学鉴定。电镜观察gaba样免疫反应性在神经分泌神经元神经末梢的定位。视上核内可见少量小的gaba能神经元,核周区仅有极少数中等大小的gaba能神经元。gaba能神经元覆盖的固有的、非gaba能小神经元也被检测到。具有gaba能轴身突触的神经细胞均匀地包裹在整个视上核的未标记的神经分泌核周围。gaba能系统似乎抑制抗利尿激素和催产素细胞。在这个区域,神经胶质细胞在反染色的细胞核周围显示出清晰的未标记的体细胞轮廓。视上核的毛细血管仅被gaba能神经膜轻微覆盖。电镜观察显示gaba能在神经分泌神经元上存在轴-体对称突触和轴-树突不对称突触。gaba样免疫反应性定位于微管和突触囊泡膜、线粒体外膜和突触前部位的内叶。许多对非免疫反应性突触沿着这些免疫反应性突触排列。
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