Fertilization induces endocytosis in Xenopus eggs

Giovanni Bernardini, Marco Ferraguti, Roberto Stipani
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引用次数: 9

Abstract

Fertilization-induced endocytosis in Xenopus eggs was shown by direct visualization of fluorescent dye in semithin sections. The eggs were incubated in a medium containing 0.1% Lucifer yellow CH for 20 min before, during and after fertilization and then fixed at different times after fertilization. The eggs incubated during or immediately after fertilization contained fluorescent vesicles in the cortex. These vesicles were mainly distributed in the animal hemisphere.

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受精诱导爪蟾卵内吞作用
利用荧光染料在半薄切片上直接显示受精诱导的爪蟾卵内吞作用。卵在含0.1%路西法黄CH的培养基中孵育20 min,受精后不同时间固定。受精期间或受精后立即孵化的卵在皮层中含有荧光囊泡。这些囊泡主要分布在动物半球。
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