The synergistic effect of hyperthermia and ethanol on changing gene expression of mouse lymphocytes.

D I Rodenhiser, J H Jung, B G Atkinson
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引用次数: 22

Abstract

Cultured mouse lymphocytes respond to a brief incubation at an elevated temperature (41-43 degrees C) with the new and (or) enhanced synthesis of a select group of polypeptides (known as heat-shock proteins, HSPs) having relative molecular masses of 110, 100, 90, 70, and 65 kilodaltons (kDa). Expression of these HSPs is dependent on new RNA synthesis. Because the synthesis of any particular HSP is dependent on the temperature and the length of time cells remain at a particular elevated temperature, synthesis of each HSP is not necessarily coordinated with the synthesis of the other HSPs. Cultured mouse lymphocytes treated with arsenite or ethanol exhibit new and (or) enhanced synthesis of HSPs with molecular masses of 110, 90, 70, and 65 kDa but do not exhibit enhanced synthesis of the 100-kDa HSP. Short-term concurrent exposure of mouse lymphocytes to an elevated temperature and a level of ethanol, which individually do not induce detectable HSP synthesis, results in the pronounced synthesis of HSPs similar to those seen following exposure to higher levels of either stress applied separately. Thus, in this study we demonstrate that hyperthermia and ethanol stress can act synergistically to affect a dramatic change in the gene expression of mouse lymphocytes.

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热疗与乙醇对小鼠淋巴细胞基因表达的协同作用。
培养的小鼠淋巴细胞对高温(41-43℃)的短暂孵育有反应,产生一组相对分子质量为110、100、90、70和65千道尔顿(kDa)的新的和(或)增强的多肽(称为热休克蛋白,HSPs)。这些热休克蛋白的表达依赖于新的RNA合成。由于任何特定热休克蛋白的合成都依赖于温度和细胞保持在特定高温下的时间长度,因此每种热休克蛋白的合成不一定与其他热休克蛋白的合成协调。用亚砷酸盐或乙醇处理培养的小鼠淋巴细胞表现出新的和(或)增强的HSP合成,其分子质量为110、90、70和65 kDa,但没有表现出100 kDa的HSP合成增强。将小鼠淋巴细胞短期同时暴露于高温和乙醇环境中,这两种环境单独不会诱导可检测到的热休克蛋白合成,但会导致热休克蛋白的明显合成,这与单独暴露于更高水平的应激环境中所见的相似。因此,在本研究中,我们证明了高温和乙醇应激可以协同作用,影响小鼠淋巴细胞基因表达的急剧变化。
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