The action of Saraca asoca Roxb. de Wilde bark on the PGH2 synthetase enzyme complex of the sheep vesicular gland.

T B Middelkoop, R P Labadie
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引用次数: 19

Abstract

Extracts of S. asoca bark and pure compounds isolated from the bark were tested for properties that might inhibit the conversion of arachidonic acid by the PGH2 synthetase. They were assayed spectrophotometrically with adrenaline as cofactor. Methanol- and ethyl acetate extracts inhibited the conversion. The observed inhibition was confirmed in an oxygraphic assay. Two procyanidin dimers from the ethyl acetate extract showed enzyme catalyzed oxidation in our assay. The ether extract of the bark was also found to contain yet unknown substances which were capable of being oxidised by the PGH2 synthetase. The combined action of the components of the bark may explain the mode of action of the drug Asoka Aristha, the main ingredient of which is the bark of S. asoca. The drug is traditionally used in Sri Lanka to treat menorrhagia.

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Saraca asoca Roxb的行动。绵羊囊腺PGH2合成酶复合物的研究。
研究了asoca树皮提取物和从树皮中分离的纯化合物对PGH2合成酶转化花生四烯酸的抑制作用。以肾上腺素为辅助因子,分光光度法测定。甲醇和乙酸乙酯提取物抑制了转化。观察到的抑制作用在氧描法中得到证实。从乙酸乙酯提取的两个原花青素二聚体在我们的实验中显示酶催化氧化。树皮的醚提取物也被发现含有未知的物质,这些物质能够被PGH2合成酶氧化。其主要成分为阿育王树皮的药物阿育王的作用机理可以通过其各成分的联合作用来解释。这种药物在斯里兰卡传统上用于治疗月经过多。
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