Immobilized enzymes at the surface of rat heart muscle mitochondria

Abstract

Of the three pairs of complementary replicas mentioned in the previous paper (1985,J. Ultrastruct. Res.91, 38–50) one pair consisted of fracture faces exposing the cytoplasmic surface of the outer surface membrane while the complementary face exposed the cytosol at the membrane surface. The latter face was particulate with randomly distributed particles in the size range of 100 to 200A˚. These particles could be shown to be located in the cytosol at the membrane surface. They qualify as particles that are loosely bound to this surface, and it is proposed that at least part of these particles consists of glycolytic enzymes.