[High resolution liquid chromatography (HPLC) analysis of the vitamin A and beta-carotene content of heat-treated precooked food].

Acta vitaminologica et enzymologica Pub Date : 1985-01-01
S Ciappellano, F Brighenti, M Porrini, G Testolin
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Abstract

Vitamin A and beta-carotene contents in 22 samples of precooked foods were determined before and after the use of various reheating techniques. The method used includes saponification by boiling with etOH and KOH, diethyl ether extraction in the presence of BHT as antioxidant, drying under controlled conditions and detection of the vitamins by HPLC. The results suggest good applicability of the procedure to food analysis and show how reheating processes can have a significant influence on the vitamin content of such foodstuffs.

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[高分辨率液相色谱(HPLC)分析热处理预熟食品中维生素A和β -胡萝卜素的含量]。
在使用各种再加热技术前后,测定了22种预煮食品样品中的维生素A和β -胡萝卜素含量。所采用的方法包括乙氧化氢和氢氧化钾煮沸皂化,在BHT作为抗氧化剂的情况下乙醚提取,在控制条件下干燥,HPLC检测维生素。结果表明,该方法对食品分析具有良好的适用性,并显示了再加热过程如何对此类食品的维生素含量产生重大影响。
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