The coelomic elements of sea urchins (Strongylocentrotus and centrostephanus—VI. Cellulose-acetate membrane electrophoresis

Comparative biochemistry and physiology Pub Date : 1970-12-01 DOI:10.1016/0010-406X(70)90557-8

Phyllis T Johnson

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引用次数: 12

Abstract

1.
1. Electrophoretic patterns of proteins and acid mucopolysaccharides found in cell-free coelomic fluid and extracts of ceolomic cells of Strongylocentrotus franciscanus, S. purpuratus, and Centrostephanus coronatus differed according to the urchin species. Most of the proteins were PAS-positive.
2.
2. In cell-free coelomic fluid, echinochrome A (6-ethyl-2,3,7-trihydroxynaphthazarin) is bound to one or more PAS-positive proteins, according to the urchin species. The same proteins occur in echinochrome-free fluids. In cell extracts echinochrome occurs in PAS-positive proteins that also have an acid-mucopolysaccharide moiety.
3.
3. Purified echinochrome A conjugated with the proteins ovomucoid and calf-thymus histone, and with macromolecules in fresh cell-free sea-urchin coelomic fluid. It did not conjugate with bovine gamma globulin, or with molecules in concentrated coelomic fluid.

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海胆(strongylocentrrotus和centrrostephanus - vi)体腔成分的研究。纤维素-醋酸膜电泳

1.1. 不同海胆品种的franciscanus、S. purpuratus和Centrostephanus coronatus的无细胞体腔液和提取液中发现的蛋白质和酸性粘多糖的电泳模式不同。大部分蛋白为pas阳性。根据海胆种类，在无细胞体腔液中，棘色素A(6-乙基-2,3,7-三羟基萘杂苷)与一个或多个pas阳性蛋白结合。同样的蛋白质也存在于无刺青色素的液体中。在细胞提取物中，棘色素出现在pas阳性蛋白中，这些蛋白也具有酸性粘多糖基团。纯化的棘皮色素A与卵泡样蛋白和小牛胸腺组蛋白结合，并与新鲜无细胞海胆体腔液中的大分子结合。它不能与牛γ球蛋白结合，也不能与浓缩体腔液中的分子结合。

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Comparative biochemistry and physiology

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