The production of hyaluronate by spontaneously established cell lines and viral transformed lines of fibroblastic origin

David Hamerman , George J. Todaro , Howard Green
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引用次数: 96

Abstract

Human diploid fibroblast strains and established mouse fibroblast lines continue to synthesize hyaluronate over many cell generations in culture. However, this property may be lost in the course of many years of serial culture of established lines as it is no longer detectable in three well-known mouse lines of fibroblastic origin, including L-cells. Transformation of the established mouse fibroblast line 3T3 by SV40 or polyoma virus and of human diploid fibroblast strains by SV40 leads in each case to a marked diminution in the rate of hyaluronate synthesis.

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由自发建立的细胞系和源自成纤维细胞的病毒转化细胞系产生的透明质酸
人二倍体成纤维细胞株和已建立的小鼠成纤维细胞系在培养的许多代细胞中继续合成透明质酸。然而,这种特性可能会在多年的连续培养过程中丢失,因为它在三种已知的成纤维细胞来源的小鼠系(包括l细胞)中不再检测到。用SV40或多瘤病毒转化已建立的小鼠成纤维细胞系3T3和用SV40转化人二倍体成纤维细胞株,在每种情况下都导致透明质酸合成速率的显著降低。
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Subject index Erratum Author index Hexosamine and acid glycosaminoglycans in human teeth Acid mucopolysaccharides of tadpole tail fin and back skin
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