African swine fever. V. Cultivation of the virus in primary pig kidney cells.

A S Greig, P Boulanger, G L Bannister
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Abstract

Six strains of African swine fever (ASF) virus were propagated in culture of primary pig kidney (PK) cells. The course of virus growth was followed by means of the fluorescent antibody staining technique. All 6 strains multiplied in the cultures, and 5 of these eventually showed cytopathic effects leading to cell death. Three of the strains were tested for pathogenicity in pigs at various passage levels. Each showed evidence of modification in virulence after a relatively few passages in PK cells. In one case modified virus produced resistance to challenge with homologous virulent virus. All strains rendered the PK cultures capable of hemadsorption of pig erythrocytes.

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非洲猪瘟。V.病毒在猪原代肾细胞中的培养。
对6株非洲猪瘟(ASF)病毒在原代猪肾(PK)细胞培养中进行了繁殖。用荧光抗体染色技术跟踪病毒的生长过程。所有6株在培养物中繁殖,其中5株最终表现出导致细胞死亡的细胞病变效应。其中三种菌株在不同传代水平上对猪进行了致病性测试。在PK细胞中相对较少的传代后,每一种都显示出毒力改变的证据。在一个病例中,修饰病毒对同源强毒病毒的攻击产生了抗性。所有菌株的PK培养物均能吸附猪红细胞的血液。
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