Biochemical characteristics of insect microsomes: NADPH oxidation by intact microsomes from the housefly, Musca domestica

Margaret D Folsom, Ernest Hodgson
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引用次数: 21

Abstract

  • 1.

    1. The oxidation of reduced pyridine nucleotides by microsomal preparations from the abdomen of the adult housefly, Musca domestica, has been examined and characterized.

  • 2.

    2. NADPH oxidation is stimulated by exogenous cytochrome-c and inhibited by CO and sulfhydryl inhibitors.

  • 3.

    3. The effect of metal ions, synergists and known substrates for microsomal mixed function oxidases is also examined.

  • 4.

    4. Added xenobiotics substrates are not required for NADPH oxidation by these preparations.

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昆虫微粒体的生化特性:家蝇完整微粒体对NADPH的氧化
1.1. 从家蝇(Musca domestica)的腹部提取的微粒体制剂对还原性吡啶核苷酸的氧化作用已被研究和鉴定。NADPH氧化受到外源性细胞色素c的刺激,并受到CO和巯基抑制剂的抑制。还研究了金属离子、增效剂和已知底物对微粒体混合功能氧化酶的影响。这些制剂不需要添加外源性底物来氧化NADPH。
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