Studies of optimal conditions for the measurement of circulating endogenous lipoprotein lipase activity

H. Engelberg
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引用次数: 3

Abstract

In 63 of 86 experiments there was a significantly greater release of free fatty acids when platelet-rich human plasma was incubated with coconut oil than when platelet-poor plasma was used. In 19 of 20 experiments fatty acid-poor albumin enhanced the lipolysis of coconut oil. The results of this study indicate that platelet-rich plasma and fatty acid-poor albumin should be used when endogenous plasma lipoprotein lipase activity is determined in vitro.

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循环内源性脂蛋白脂肪酶活性测定的最佳条件研究
在86个实验中的63个实验中,当富含血小板的人血浆与椰子油一起孵育时,游离脂肪酸的释放量明显大于使用血小板较少的血浆时的释放量。在20个实验中的19个中,缺乏脂肪酸的白蛋白促进了椰子油的脂肪分解。本研究结果表明,在体外测定内源性血浆脂蛋白脂肪酶活性时,应采用富血小板血浆和贫脂肪酸白蛋白。
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