Effects of temperature on Drosophila—VII. Glutamate-aspartate transaminase activity

Mary Jo Burr, Alice S. Hunter
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引用次数: 5

Abstract

  • 1.

    1. Glutamate-aspartate transaminase activity in homogenates of young adults is reported for stocks of Drosophila melanogaster, D. immigrans, D. pseudoobscura and D. willistoni grown at 15 and 25°C.

  • 2.

    2. Female D. melanogaster acclimated at 25°C, have lower glutamate-aspartate transaminase activity, measured at 20°C, than do those grown at the “normal” temperature of 15°C.

  • 3.

    3. Glutamate-aspartate transaminase activity of young adult males and females of D. immigrans acclimated at 25°C is lowe than that of flies grown at 15°C when measured at 20°C.

  • 4.

    4. Transaminase activity does not vary with acclination temperature in young adults of the two stenothermal species, D. pseudoobscura and D. willistoni.

  • 5.

    5. The data reported support the theory that eurythermal species at Drosophila have greater capacity for physiological adaptation than do stenothermal species. It is theorized that respiratory control in Drosophila may be related to rates of amino acid conversion and the level of substrates entering the Krebs cycle.

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温度对果蝇的影响。谷氨酸-天冬氨酸转氨酶活性
1.1. 据报道,在15°c和25°c条件下生长的黑胃果蝇(Drosophila melanogaster)、移民果蝇(d.d migrans)、伪眼果蝇(d.p oobscura)和威尔氏果蝇(d.w willlistoni)的年轻成虫匀浆中谷氨酸-天冬氨酸转氨酶活性为2.2。在25°C环境下驯化的雌性黑胃d.m anogaster,在20°C环境下的谷氨酸-天冬氨酸转氨酶活性比在“正常”温度15°C环境下生长的要低3.3。在20℃条件下测定,25℃条件下驯化的迁徙家蝇雌雄成虫谷氨酸-天冬氨酸转氨酶活性低于15℃条件下生长的家蝇4.4。在两种低温物种(pseudoobscura D.和willistond .)的年轻成虫中,转氨酶活性不随气候温度变化。报告的数据支持了这样的理论,即果蝇的低温物种比低温物种具有更大的生理适应能力。从理论上讲,果蝇的呼吸控制可能与氨基酸转化率和进入克雷布斯循环的底物水平有关。
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