Studies on the kynurenine transaminase of Schistocerca gregaria forsk. (Orthoptera, acrididae)

Comparative biochemistry and physiology Pub Date : 1970-12-01 DOI:10.1016/0010-406X(70)90559-1

S Pinamonti, A Petris, M Miliani

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引用次数: 14

Abstract

1.
1. The kynurenine transaminase of different tissues and of the haemolymph of adult Schistocerca gregaria was studied. It is present only in the homogenate of the locust fat body.
2.
2. It is active both on kynurenine and 30H-kynurenine. The K_m is 4·4 × 10⁻⁴ M for kynurenine and 1·5 × 10⁻³ M for 30H-kynurenine. For both substrates the optinum pH is 8.
3.
3. Pyruvic and oxalacetic acids are slightly more active than α-ketoglutaric acid as—NH₂ acceptors. High concentrations of ketoacids are not inhibitory. Pyridoxal phosphate is necessary for the enzyme acitivity.
4.
4. Locust kynurenine transaminase is similar to the same enzyme of mammals; one of the differences is that for the locust enzyme the best—NH₂ acceptor is pyruvic acid.

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浙江血吸虫叶犬尿氨酸转氨酶的研究。(acrididae直翅目)

1.1. 研究了成虫血淋巴和不同组织的犬尿氨酸转氨酶。它只存在于蝗虫脂肪体的匀浆中。它对犬尿氨酸和30h -犬尿氨酸都有活性。犬尿氨酸的Km为4.4 × 10−4 M, 30h犬尿氨酸的Km为1.5 × 10−3 M。两种底物的最佳pH均为8.3.3。丙酮酸和草酸作为nh2受体的活性略高于α-酮戊二酸。高浓度的酮酸没有抑制作用。磷酸吡哆醛对酶的活性是必需的。蝗虫犬尿氨酸转氨酶与哺乳动物相似;其中一个区别是，对于蝗虫酶来说，最好的nh2受体是丙酮酸。

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Comparative biochemistry and physiology

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