Enzymatic studies of chondroitin sulphates in human arterial tissue

K. Murata, T. Harada, K. Okubo
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引用次数: 27

Abstract

  • (1)

    Isomeric chondroitin sulphates of human arterial tissues were analysed by enzymatic methods with chondroitinases and sulphatases.

  • (2)

    The enzymatic methods furnished a linear relation between the amount of arterial tissue sample and the yield of chondroitin sulphate isomers.

  • (3)

    The present data showed that chondroitin sulphate C is a major component in arterial tissue, followed by chondroitin sulphate B as a lesser component. Evidence is presented for the presence of chondroitin sulphate A in approximately equal amount to chondroitin sulphate B. This finding was supported by chromatographic separation of the degraded products of chondroitin sulphate isomers with chondroitinases.

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人动脉组织硫酸软骨素的酶促研究
(1)用硫酸软骨素酶和软骨素酶对人动脉组织的硫酸软骨素异构体进行了酶促分析。(2)酶促分析结果表明,动脉组织样品的数量与硫酸软骨素异构体的产率呈线性关系。(3)目前的数据表明,硫酸软骨素C是动脉组织中的主要成分,其次是硫酸软骨素B。有证据表明,硫酸软骨素A与硫酸软骨素b的含量大致相等。这一发现得到了用软骨素酶对硫酸软骨素异构体降解产物进行色谱分离的支持。
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