Hageman factor, a novel sialoglycoprotein with esterase activity

Abstract

A novel esterase has been isolated from bovine plasma in a highly purified form. According to electrophoresis and ultracentrifugal sedimentation patterns it represents a homogeneous 7.1 S sialoglycoprotein being composed of 5.9% neutral carbohydrates, 4.8% aminosugars and 4.4% sialic acid. In catalytic amounts it normalizes the prolonged clotting time of plasma deficient in Hageman factor and exhibits a strong esterase activity towards $\text{N-}\text{benzoyl-}\text{l}\text{-arginine}$ ethyl ester.