{"title":"Density-gradient banding of denatured deoxyribonucleic acid in cesium sulphate","authors":"Donald J. Cummings, Lee Mondale","doi":"10.1016/0926-6585(66)90311-6","DOIUrl":null,"url":null,"abstract":"<div><p>A new continuous-source, monochromatic ultraviolet optical system designed for use in the Spinco Model-E ultracentrifuge was constructed and its properties described. Using this system, the flotation density of native and denatured DNA from various T-bacteriophages in Cs<sub>2</sub>SO<sub>4</sub> was determined. It was found that all the denatured DNA's examined gave rise to two density bands, except for T6 DNA which possibly had three bands, in Cs<sub>2</sub>SO<sub>4</sub> but not in CsCl. The relevance of these findings to the data of others was discussed.</p></div>","PeriodicalId":100158,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Biophysics including Photosynthesis","volume":"120 3","pages":"Pages 448-453"},"PeriodicalIF":0.0000,"publicationDate":"1966-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0926-6585(66)90311-6","citationCount":"10","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biochimica et Biophysica Acta (BBA) - Biophysics including Photosynthesis","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/0926658566903116","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 10
Abstract
A new continuous-source, monochromatic ultraviolet optical system designed for use in the Spinco Model-E ultracentrifuge was constructed and its properties described. Using this system, the flotation density of native and denatured DNA from various T-bacteriophages in Cs2SO4 was determined. It was found that all the denatured DNA's examined gave rise to two density bands, except for T6 DNA which possibly had three bands, in Cs2SO4 but not in CsCl. The relevance of these findings to the data of others was discussed.
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