Environmental effects on the ultraviolet absorption spectrum of tyrosine

Biochimica et Biophysica Acta (BBA) - Biophysics including Photosynthesis Pub Date : 1966-09-05 DOI:10.1016/0926-6585(66)90031-8

H. Grinspan, J. Birnbaum, J. Feitelson

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引用次数: 16

Abstract

The influence of a variety of mono- and polybasic alcohols, ethers and acids on the electronic absorption spectrum of tyrosine in aqueous solution was investigated.

A shift towards longer wavelength in the first ( $L_{b}$ ) and the second ( $L_{a}$ ) band is observed as a function of solvent composition. The results are compared with those obtained for phenylalanine in similar solvent systems. It is found that the effects can be discussed in terms of hydrogen bond formation between tyrosine, acting as proton donor, and the various solvent components as proton acceptors. It is suggested that the changes in spectral properties due to hydrogen bonding may be attributed to a modification in the substituent effect of the hydroxyl group on the spectrum of the aromatic system.

The implications of these effects in polypeptide conformation studies are briefly mentioned.

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环境对酪氨酸紫外吸收光谱的影响

研究了多种单、多碱醇、醚和酸对水溶液中酪氨酸电子吸收谱的影响。在第一波段(Lb)和第二波段(La)中观察到向较长波长的偏移，这是溶剂组成的函数。并与同类溶剂体系中苯丙氨酸的测定结果进行了比较。研究发现，这种效应可以从酪氨酸作为质子供体和各种溶剂组分作为质子受体之间形成氢键的角度来讨论。结果表明，氢键引起的光谱性质变化可能是由于羟基取代基对芳系光谱的影响发生了改变。简要地提到了这些效应在多肽构象研究中的意义。

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Biochimica et Biophysica Acta (BBA) - Biophysics including Photosynthesis

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