A simplified immunofluorescence technique for antibody to varicella-zoster membrane antigen (FAMA).

Biken journal Pub Date : 1984-03-01
K Baba, M Yoshida, A Tawa, H Yabuuchi, K Maeda, M Takahashi
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Abstract

Indirect fluorescent antibody to varicella-zoster membrane antigens (FAMA) was measured by a new technique. The procedure gives rapid, sensitive and accurate results and is suitable for use in diagnosis or screening of susceptibility to varicella-zoster virus (VZV) infection. The test procedure was simplified by using Terasaki tissue culture plates for the reaction and for direct observation by fluorescence microscopy. Preparations of VZV-infected Vero cells stored in liquid nitrogen could be used as antigen in this FAMA-test.

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水痘-带状疱疹膜抗原(FAMA)抗体的简化免疫荧光技术。
采用新方法测定了水痘-带状疱疹膜抗原间接荧光抗体(FAMA)。该程序可提供快速、敏感和准确的结果,适用于水痘-带状疱疹病毒(VZV)感染的诊断或易感性筛查。通过使用Terasaki组织培养板进行反应和荧光显微镜直接观察,简化了测试程序。vzv感染的Vero细胞在液氮中保存的制剂可作为fama试验的抗原。
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