{"title":"Rapid detection of pathognomonic blood cells in patients with infectious mononucleosis.","authors":"K Grossgebauer, H D Pohle","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>Veneous blood-citrate samples of patients with infectious mononucleosis were stained with DAPI, a newer fluorochrome (4',6-diamidino-2-phenylindole) to demonstrate the \"large atypical lymphocytes\" associated with this disease. As a result of the capacity of DAPI to stain DNA and certain acid mucopolysaccharides, a rapid detection of the pathognomonic white blood cells could be achieved. Most of them revealed the well known indented or lobulated nuclei and vacuolated (foamy) cytoplasm. Others exhibited kidney-shaped nuclei, often filled with yellow-fluorescent tiny granula. In our view, the rapid, specific and sensitive DAPI-technique for detecting pathognomonic blood cells in patients with infectious mononucleosis can be considered an improved microscopic method.</p>","PeriodicalId":76158,"journal":{"name":"Microscopica acta","volume":"86 4","pages":"289-93"},"PeriodicalIF":0.0000,"publicationDate":"1982-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Microscopica acta","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
Veneous blood-citrate samples of patients with infectious mononucleosis were stained with DAPI, a newer fluorochrome (4',6-diamidino-2-phenylindole) to demonstrate the "large atypical lymphocytes" associated with this disease. As a result of the capacity of DAPI to stain DNA and certain acid mucopolysaccharides, a rapid detection of the pathognomonic white blood cells could be achieved. Most of them revealed the well known indented or lobulated nuclei and vacuolated (foamy) cytoplasm. Others exhibited kidney-shaped nuclei, often filled with yellow-fluorescent tiny granula. In our view, the rapid, specific and sensitive DAPI-technique for detecting pathognomonic blood cells in patients with infectious mononucleosis can be considered an improved microscopic method.
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