{"title":"Carbodiimide and formalin as fixatives for the TP-levanol fast cyanine 5RN-method for staining myosin-like proteins in rat tissues.","authors":"E van Pelt-Verkuil","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>Apart from the applicability of the tannic acid-phosphomolybdic acid-Levanol-Fast Cyanine 5RN method for the identification of muscle myosins in Carnoy-fixed tissues, this technique can also be applied on tissue sections fixed with formalin or carbodiimide. The use of carbodiimide as a fixative offers, in general, a superior staining of the tissues with safranine and Levanol. Using a slightly modified procedure, reduction of staining time in Levanol, moreover, allows a differentiation between cells containing different amounts of myosin. This is illustrated by means of a number of rat tissues.</p>","PeriodicalId":76158,"journal":{"name":"Microscopica acta","volume":"86 4","pages":"323-38"},"PeriodicalIF":0.0000,"publicationDate":"1982-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Microscopica acta","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
Apart from the applicability of the tannic acid-phosphomolybdic acid-Levanol-Fast Cyanine 5RN method for the identification of muscle myosins in Carnoy-fixed tissues, this technique can also be applied on tissue sections fixed with formalin or carbodiimide. The use of carbodiimide as a fixative offers, in general, a superior staining of the tissues with safranine and Levanol. Using a slightly modified procedure, reduction of staining time in Levanol, moreover, allows a differentiation between cells containing different amounts of myosin. This is illustrated by means of a number of rat tissues.
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