Mał, lgorzata Komorowska , Maria Koter , Grzegorz Bartosz , Jan Gomuł , lkiewicz
{"title":"The effects of glutaraldehyde and osmium tetroxide on the erythrocyte membrane","authors":"Mał, lgorzata Komorowska , Maria Koter , Grzegorz Bartosz , Jan Gomuł , lkiewicz","doi":"10.1016/0005-2736(82)90154-7","DOIUrl":null,"url":null,"abstract":"<div><div>A nitroxide spin label probe technique was applied to study the interaction between glutaraldehyde or osmium tetroxide (OsO<sub>4</sub>) and the membranes of horse erythrocytes, ghosts and liposomes prepared from erythrocyte lipids. Two major conclusions have been established: (1) Reaction of the fixation reagents with the membrane is selective. OsO<sub>4</sub> reacts predominantly with lipids and glutaraldehyde with membrane proteins. (2) The lipid-protein interactions change after pretreatment by OsO<sub>4</sub> or glutaraldehyde.</div></div>","PeriodicalId":8831,"journal":{"name":"Biochimica et biophysica acta. Biomembranes","volume":"686 1","pages":"Pages 94-98"},"PeriodicalIF":2.5000,"publicationDate":"1982-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biochimica et biophysica acta. Biomembranes","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/0005273682901547","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
A nitroxide spin label probe technique was applied to study the interaction between glutaraldehyde or osmium tetroxide (OsO4) and the membranes of horse erythrocytes, ghosts and liposomes prepared from erythrocyte lipids. Two major conclusions have been established: (1) Reaction of the fixation reagents with the membrane is selective. OsO4 reacts predominantly with lipids and glutaraldehyde with membrane proteins. (2) The lipid-protein interactions change after pretreatment by OsO4 or glutaraldehyde.
期刊介绍：
BBA Biomembranes has its main focus on membrane structure, function and biomolecular organization, membrane proteins, receptors, channels and anchors, fluidity and composition, model membranes and liposomes, membrane surface studies and ligand interactions, transport studies, and membrane dynamics.
京公网安备 11010802042870号
京ICP备2023020795号-1
分享
求助内容：
应助结果提醒方式：
扫码关注我们
