Cyclic AMP-dependent protein kinase content of murine splenic T and B lymphocytes and non-lymphoid tissues.

Abstract

Cyclic AMP-dependent protein kinase activity has been measured in DEAE-cellulose elution profiles of highly enriched murine splenic T and B Lymphocytes. B Lymphocytes were found to contain about 10% of the amount of enzyme activity associated with T lymphocytes and several non-lymphoid tissues. Cyclic AMP-independent casein kinase activities were equivalent in T and B lymphocytes. Lymphocytes contained predominantly type I isozyme as opposed to several non-lymphoid tissues which all contained predominantly the type II form.