The application of a sepharose bead immunofluorescence assay and a solid-phase radioimmunoassay to the bovine leukemia virus system.

Acta biologica et medica Germanica Pub Date : 1982-01-01
H Fiebach, W Uckert, B Micheel
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Abstract

Several fluorescence assays with bovine leukemia virus (BLV) conjugated to activated Sepharose 4B were used for the detection of BLV and anti-BLV antibodies. These tests were compared with a solid-phase radioimmunoassay and found to be in the same sensitivity range. Sepharose bead immunofluorescence assay and solid-phase radioimmunoassay can be applied to the diagnosis of BLV infection in cattle.

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糖珠免疫荧光法和固相放射免疫法在牛白血病病毒系统中的应用。
用活化的Sepharose 4B偶联的牛白血病病毒(BLV)荧光法检测BLV和抗BLV抗体。这些试验与固相放射免疫测定法比较,发现在相同的灵敏度范围内。糖珠免疫荧光法和固相放射免疫法可用于牛BLV感染的诊断。
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