{"title":"An easy method for comparing antibody affinities to related antigens.","authors":"B Aasted, B Avery","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>Four different Aleutian disease virus (ADV) isolates were coated to 1/4\" polystyrene balls. The binding capacity of these balls against different mouse hybridoma antibodies prepared against each of the ADV isolates was investigated in two ways. The different ADV-coated balls were either mixed in a tube (competition between balls for antibody), or they were allowed to react separately with antibody (no competition). The difference in competitive and non-competitive binding values allows a comparison of antibody affinity against the four ADV isolates. Examples are given, where two hybridoma antibodies react better with the Danish isolate than with the three American isolates.</p>","PeriodicalId":77653,"journal":{"name":"Acta pathologica, microbiologica, et immunologica Scandinavica. Section C, Immunology","volume":"91 1","pages":"65-7"},"PeriodicalIF":0.0000,"publicationDate":"1983-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Acta pathologica, microbiologica, et immunologica Scandinavica. Section C, Immunology","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
Four different Aleutian disease virus (ADV) isolates were coated to 1/4" polystyrene balls. The binding capacity of these balls against different mouse hybridoma antibodies prepared against each of the ADV isolates was investigated in two ways. The different ADV-coated balls were either mixed in a tube (competition between balls for antibody), or they were allowed to react separately with antibody (no competition). The difference in competitive and non-competitive binding values allows a comparison of antibody affinity against the four ADV isolates. Examples are given, where two hybridoma antibodies react better with the Danish isolate than with the three American isolates.
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