Demonstration of Aujeszky's disease virus antigen in trypsin-digested paraffin-embedded tissue sections by immunofluorescence.

M Narita, S Inui, M Kobayashi, A Fukusho
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Abstract

After trypsin digestion of buffered formalin-fixed, paraffin-embedded brain section, immunofluorescent indication of Aujeszky's disease virus antigen was successful in experimentally infected cow and goat. This result was comparable to those obtained with light and electron microscopic examinations. This method will be diagnostically useful where fresh-frozen tissue is not available.

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免疫荧光法在胰蛋白酶消化石蜡包埋组织切片上显示奥杰斯基氏病病毒抗原。
经胰蛋白酶消化缓冲的福尔马林固定石蜡包埋脑切片后,在实验感染的牛和山羊中成功地获得了奥杰斯基病病毒抗原的免疫荧光指示。这一结果与光镜和电镜检查所得的结果相当。这种方法将是诊断有用的新鲜冷冻组织是不可用的。
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