Secretion of IgM-rheumatoid factor and IgM by blood lymphocytes in rheumatoid arthritis.

Acta pathologica, microbiologica, et immunologica Scandinavica. Section C, Immunology Pub Date : 1983-02-01

C Heilmann, J Petersen, V Andersen, O J Bjerrum, B Dinesen

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Abstract

A direct plaque forming cell (PFC) assay for the detection of complement fixing IgM-rheumatoid factor (RF) secreting lymphocytes was evaluated. The specificity of the RF-PFC assay was demonstrated by the inhibitory action of exogenous human IgG. The sensitivity of the RF-PFC assay was similar to that of a reverse PFC assay detecting all cells secreting IgM (IgM-PFC). In blood from 75% of seropositive patients with rheumatoid arthritis (RA) RF-PFC were demonstrated, median value 6.8 (range: 0.4-1477) RF-PFC/10(6) mononuclear cells. Practically no RF-PFC were detected in seronegative RA patients and controls. Despite the fact that most IgM-RF undoubtedly is produced outside the blood, a positive correlation was found between the number of RF-PFC and the Waaler-Rose titer in serum. The number of circulating IgM secreting cells did not differ significantly between seropositive RA patients, seronegative RA patients and controls. Comparison of RF-PFC and IgM-PFC in seropositive patients revealed that in mean 7% of IgM-secreting cells in blood secrete IgM-RF.

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类风湿关节炎患者外周血淋巴细胞分泌IgM-类风湿因子及IgM。

评估了直接斑块形成细胞(PFC)检测补体固定igm -类风湿因子(RF)分泌淋巴细胞的方法。外源人IgG的抑制作用证明了RF-PFC检测的特异性。RF-PFC检测的灵敏度与检测所有分泌IgM的细胞(IgM-PFC)的反向PFC检测相似。在75%的类风湿性关节炎(RA)血清阳性患者的血液中显示RF-PFC，中位值为6.8(范围:0.4-1477)RF-PFC/10(6)个单个核细胞。在血清阴性的RA患者和对照组中几乎没有检测到RF-PFC。尽管大多数IgM-RF无疑是在血液外产生的，但我们发现RF-PFC的数量与血清中的Waaler-Rose效价呈正相关。血清阳性RA患者、血清阴性RA患者和对照组之间循环IgM分泌细胞的数量无显著差异。血清阳性患者的RF-PFC和IgM-PFC比较显示，血液中平均7%的igm分泌细胞分泌IgM-RF。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Acta pathologica, microbiologica, et immunologica Scandinavica. Section C, Immunology

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