{"title":"Comparison of enzyme-linked immunosorbent assay and radioimmunoassay for determination of anti-pneumococcal polysaccharide antibodies.","authors":"F K Pedersen, J Henrichsen, G Schiffman","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>Antibodies against pneumococcal capsular polysaccharide types 1, 3, 6A, 7F, 8 and 9N were determined before and 4 weeks after pneumococcal vaccination by both enzyme-linked immunosorbent assay (ELISA) and radioimmunoassay (RIA) in 49 children, 42 of whom had been splenectomized. Using linear regression analysis a significant correlation between total antibody concentrations determined by ELISA and RIA was found for all 6 antigens (p less than 0.001 for each). The degree of correlation varied between types, antibodies against type 3 showing the best concordance, those against type 6A the poorest. In 84% of the samples assayed both the RIA and the ELISA determined antibody concentrations were either above (55%) or below (29%) the hypothetical protective antibody concentration.</p>","PeriodicalId":77653,"journal":{"name":"Acta pathologica, microbiologica, et immunologica Scandinavica. Section C, Immunology","volume":"91 4","pages":"251-5"},"PeriodicalIF":0.0000,"publicationDate":"1983-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Acta pathologica, microbiologica, et immunologica Scandinavica. Section C, Immunology","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
Antibodies against pneumococcal capsular polysaccharide types 1, 3, 6A, 7F, 8 and 9N were determined before and 4 weeks after pneumococcal vaccination by both enzyme-linked immunosorbent assay (ELISA) and radioimmunoassay (RIA) in 49 children, 42 of whom had been splenectomized. Using linear regression analysis a significant correlation between total antibody concentrations determined by ELISA and RIA was found for all 6 antigens (p less than 0.001 for each). The degree of correlation varied between types, antibodies against type 3 showing the best concordance, those against type 6A the poorest. In 84% of the samples assayed both the RIA and the ELISA determined antibody concentrations were either above (55%) or below (29%) the hypothetical protective antibody concentration.
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