Isolation of the ST Ent plasmid specific bacteriophage M124.

Biken journal Pub Date : 1983-09-01
P Soedarmono, A Matsushiro, K Hoshi, Sujudi
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引用次数: 0

Abstract

For simple detection of enterotoxigenic E. coli from patients, attempts were made to find a bacteriophage that specifically proliferate on these bacteria. As a result phage M124 was isolated. Analysis of plasmid DNA together with detection of ST enterotoxin showed that this phage can specifically attack E. coli carrying the ST Ent plasmid (80 Mdalton). These results indicate that phage M124 is very useful for detection of ST enterotoxigenic bacteria.

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质粒特异性噬菌体M124的分离。
为了从病人身上简单地检测出产肠毒素的大肠杆菌,人们试图找到一种能在这些细菌上特异性增殖的噬菌体。结果分离出噬菌体M124。质粒DNA分析和ST肠毒素检测表明,该噬菌体能特异性攻击携带ST Ent质粒(80 Mdalton)的大肠杆菌。这些结果表明噬菌体M124对ST肠毒素细菌的检测是非常有用的。
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