[Selective citrate-adonitol medium for the isolation of various strains of Escherichia coli K99].

Annales de microbiologie Pub Date : 1984-07-01
P Pohl, P Lintermans, M C Vandergheynst, K Van Muylem, C Schlicker, G Van Robaeys
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Abstract

Simmons citrate agar supplemented with 0.2% adonitol (SCA medium) was used as a differential medium for some enterotoxigenic Escherichia coli: all strains with either a cryptic K99 antigen or a glucose-dependent K99 antigen and 70% of strains with a glucose-independent K99 antigen gave yellow colonies on SCA medium. Other faecal bacteria did not grow or else developed as blue colonies.

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[选择柠檬酸盐-甘油三醇培养基分离大肠杆菌K99菌株]。
用添加0.2% adonitol (SCA培养基)的Simmons citrate琼脂作为某些产肠毒素大肠杆菌的差示培养基:所有具有隐性K99抗原或葡萄糖依赖性K99抗原的菌株和70%具有葡萄糖非依赖性K99抗原的菌株在SCA培养基上形成黄色菌落。其他的粪便细菌没有生长或发展成蓝色菌落。
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