M Itoh, J Ishihara, A Itagaki, K Taniguchi, K Miyai, T Kurimura
{"title":"Enzyme immunoassay of HBeAg employing beta-D-galactosidase.","authors":"M Itoh, J Ishihara, A Itagaki, K Taniguchi, K Miyai, T Kurimura","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>An enzyme-linked immunosorbent assay (ELISA) system for hepatitis B e antigen (HBeAg) was developed employing beta-D-galactosidase conjugated with antibody to HBeAg (anti-HBe) and using m-maleimidobenzoyl-N-hydroxysuccinimide ester as the coupling reagent. The experimental conditions for quantitative assay of HBeAg were determined. The presence of rheumatoid factor in test sera did not affect the results. This assay system is more sensitive than the micro-Ouchterlony method and as sensitive as radioimmunoassay. The use of beta-D-galactosidase for ELISA in the field of virology is recommended.</p>","PeriodicalId":8767,"journal":{"name":"Biken journal","volume":"26 3","pages":"121-5"},"PeriodicalIF":0.0000,"publicationDate":"1983-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biken journal","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
An enzyme-linked immunosorbent assay (ELISA) system for hepatitis B e antigen (HBeAg) was developed employing beta-D-galactosidase conjugated with antibody to HBeAg (anti-HBe) and using m-maleimidobenzoyl-N-hydroxysuccinimide ester as the coupling reagent. The experimental conditions for quantitative assay of HBeAg were determined. The presence of rheumatoid factor in test sera did not affect the results. This assay system is more sensitive than the micro-Ouchterlony method and as sensitive as radioimmunoassay. The use of beta-D-galactosidase for ELISA in the field of virology is recommended.
采用β - d -半乳糖苷酶偶联抗乙型肝炎e抗原(HBeAg)抗体，以间马来酰亚胺苯甲酰- n -羟基琥珀酰亚胺酯为偶联试剂，建立了乙型肝炎e抗原(HBeAg)酶联免疫吸附检测系统。确定了HBeAg定量测定的实验条件。检测血清中类风湿因子的存在不影响结果。该检测系统比微奥氏法更灵敏，与放射免疫测定法一样灵敏。推荐在病毒学领域使用β - d -半乳糖苷酶进行ELISA检测。
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