A qualitative ultrastructural study of the intercellular spaces between epithelial cells treated in vivo with DMBA.

F H White, K Gohari
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引用次数: 14

Abstract

One of the features of epithelial dysplasia at the histological level is known as "loss of cellular adherence" in which adjacent epithelial cells appear more widely separated from each other than in normal tissues. In this study we examine the effects of the carcinogen DMBA on the epithelium of the hamster cheek-pouch with particular emphasis on the dimensions of the intercellular spaces. DMBA-induced lesions were processed for electron microscopy and assigned to hyperplasia, dysplasia and carcinoma groups, using defined criteria on toluidine blue-stained 1 micron Araldite sections. Untreated pouches were used as a control. At the light-microscopical level, intercellular spaces in hyperplastic epithelium appeared similar to those present in untreated tissue but increased progressively in dysplastic and carcinomatous lesions. Spaces were generally wider between basal and spinous cells than between granular cells, although wide variations were observed between tissue blocks demonstrating similar histological features and also within adjacent areas of the same block. At the ultrastructural level, untreated and hyperplastic tissue showed only occasional focal separations of adjacent plasma membranes; these spaces were more frequent between cells of lower strata. In sections from dysplasias and carcinomas, spaces were always extensive and were occupied by numerous villous or foliate membrane-bound cytoplasmic extensions. These were often attached to each other by desmosomes of apparently normal morphology but of a lower frequency than in untreated epithelium. The increased epithelial separation as indicated by the increased intercellular spaces during chemical carcinogenesis may be a result of any or all of the following factors: desmosomal disruption or their failure to develop; the production of cell-surface molecules which are less adhesive; inflammatory oedema and direct alterations on intercellular junctions and cell-surface components by infiltrating inflammatory cells.

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在体内用DMBA处理上皮细胞间细胞间隙的定性超微结构研究。
在组织学水平上,上皮发育不良的特征之一是“细胞粘附性丧失”,相邻上皮细胞之间的距离比正常组织更大。在这项研究中,我们研究了致癌物DMBA对仓鼠颊袋上皮的影响,特别强调细胞间隙的尺寸。在甲苯胺蓝染色的1微米Araldite切片上,用确定的标准对dba诱导的病变进行电镜处理,并将其分为增生、不典型增生和癌组。未处理的小袋作为对照。在光镜下,增生性上皮的细胞间隙与未治疗组织相似,但在发育不良和癌变病变中逐渐增加。基底细胞和棘细胞之间的间隙通常比颗粒细胞之间的间隙更宽,尽管在具有相似组织学特征的组织块之间以及在同一块的相邻区域内观察到很大的差异。在超微结构水平上,未经治疗和增生的组织仅偶有邻近质膜的局灶性分离;这些空隙在下层细胞间更为常见。在发育不良和癌的切片中,间隙总是很宽,被许多绒毛状或叶状膜结合的细胞质延伸所占据。这些细胞通常通过桥粒相互连接,其形态明显正常,但频率低于未经处理的上皮。化学癌变过程中细胞间隙增大所显示的上皮细胞分离增加可能是下列任何或所有因素的结果:桥粒体破坏或其发育失败;产生黏性较差的细胞表面分子;炎性水肿及浸润炎性细胞对细胞间连接和细胞表面成分的直接改变。
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