[pH-induced inactivation of glycerol dehydrogenase from Bacillus megaterium].

A Ganzhorn, M Scharschmidt, G Pfleiderer
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Abstract

The kinetic of the reversible dissociation of glycerol dehydrogenase from Bacillus megaterium in slightly alkaline medium was measured by biochemical, chemical and physical methods. The dissociation is followed by changes in the secondary structure and can be prevented by addition of NAD or increased potassium chloride concentration. Crosslinking by suberimidate, but not by monofunctional imido esters, shows a high stabilization against alkali, urea or heat inactivation caused by hindrance of dissociation.

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[ph诱导的巨芽孢杆菌甘油脱氢酶失活]。
采用生化、化学和物理等方法,研究了微碱性培养基中巨型芽孢杆菌甘油脱氢酶的可逆解离动力学。解离后二级结构发生变化,可以通过添加NAD或增加氯化钾浓度来防止。亚酰亚咪酯的交联,而不是单功能亚胺酯的交联,对碱、尿素或由解离障碍引起的热失活具有很高的稳定性。
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