Identification of a disialoganglioside (GD1a) containing terminal N-acetyl-9-O-acetylneuraminic acid in rat erythrocytes.

Hoppe-Seyler's Zeitschrift fur physiologische Chemie Pub Date : 1984-10-01 DOI:10.1515/bchm2.1984.365.2.1247

D C Gowda, G Reuter, A K Shukla, R Schauer

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引用次数: 26

Abstract

Gangliosides containing 350 micrograms of sialic acids were isolated from 2.85 X 10(11) rat erythrocytes and found to be mainly composed of GD1a and an unknown alkali-labile species which was converted to GD1a after treatment with ammonia. Smaller amounts of GM1 and Fuc-GM1 were also present. Identification of the sialic acids of the novel species by thin-layer chromatography, high performance liquid chromatography and gas-liquid chromatography--mass spectrometry revealed the presence of both N-acetylneuraminic acid and N-acetyl-9-O-acetyl-neuraminic acid in about equimolar amounts. Incubation of the isolated ganglioside with Vibrio cholerae sialidase released N-acetyl-9-O-acetyl-neuraminic acid. Non O-acetylated GM1 was identified as the only remaining ganglioside by thin-layer chromatography. Thus this novel ganglioside has the following structure: Neu5,9Ac2 alpha 2-3Gal beta 1-3GalNAc beta 1-4(Neu5Ac alpha-2-3)Gal beta 1-4Glc beta 1-1'Cer.

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大鼠红细胞中含有末端n -乙酰-9- o -乙酰神经氨酸的双胞脂苷(GD1a)的鉴定。

从2.85 × 10(11)只大鼠红细胞中分离到含有350微克唾液酸的神经节苷，主要由GD1a和一种未知的碱不稳定物质组成，该物质经氨处理后转化为GD1a。少量的GM1和Fuc-GM1也存在。通过薄层色谱、高效液相色谱和气液相色谱-质谱法对该新种唾液酸进行鉴定，发现n-乙酰神经氨酸和n-乙酰-9- o -乙酰神经氨酸的含量约为等摩尔。分离的神经节苷脂与霍乱弧菌唾液酸酶孵育释放n -乙酰-9- o -乙酰-神经氨酸。薄层色谱法鉴定非o -乙酰化GM1为唯一残留的神经节苷脂。因此，这种新型神经节苷脂具有以下结构:neu5,9ac2 α 2-3Gal β 1-3GalNAc β 1-4(Neu5Ac α -2-3)Gal β 1-4Glc β 1-1'Cer。

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Hoppe-Seyler's Zeitschrift fur physiologische Chemie

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