Behaviour of a Trypanosoma brucei brucei stock (STIB 348C) in mice. 4. Different course of primary parasitemia in trypanosome variants of different virulence.

Abstract

In three types of trypanosomes with low virulence, trypanosome numbers increased by a factor of 18-21 per day on average during prepatency and by a factor of 70-219 on average from the first to second day of patency. By contrast, a very virulent trypanosome variant showed an average increase of trypanosome numbers per day by 32 and 28 during prepatency and early patency, respectively. --In detailed studies, trypanosomes of low virulence exhibited a rapidly rising parasitemia in early patency which lasted for 20-30 hours and was followed by a plateau of slowly rising and falling parasitemia. Trypanosomes of high virulence showed a constant logarithmic increase of their numbers, slowing down at concentrations above antilog 5.5 per microliters of blood. In mild trypanosomes with peak parasitemias of antilog 5-5.7 per microliters of blood, after low dose infections the primary parasitemia was abruptly terminated after 70-100 hours of patency, obviously by the action of antibody. After massive infections, the parasitemia was terminated at 109-122 hours after infection. --In trypanosomes with higher peak parasitemias, primary parasitemias were seen to last longer, in some cases for 7 to 12 days. --Mice infected with low doses of highly virulent trypanosomes died with high parasitemias after some 60-90 hours of patency, before antibodies could normally become effective. After massive infections they died at 40-60 hours after infection. There is clearly no need to invoke non-immunogenicity of these trypanosomes or immunosuppression by these trypanosomes to explain this course of the infection.