{"title":"Ecdysteroid metabolism by soluble enzymes from an insect. Metabolic relationships between 3 beta-hydroxy-, 3 alpha-hydroxy- and 3-oxoecdysteroids.","authors":"C Blais, R Lafont","doi":"10.1515/bchm2.1984.365.2.809","DOIUrl":null,"url":null,"abstract":"<p><p>The formation and fate of 3-dehydroecdysteroids were studied in vitro in tissue extracts from Pieris brassicae (Lepidoptera). Three cytosolic enzymes are involved: an ecdysone oxidase converts 3 beta-hydroxyecdysteroids into 3-dehydroecdysteroids, and two different reductases, requiring NADPH as cofactor, transform 3-dehydroecdysteroids into the 3 alpha-hydroxy- or the 3 beta-hydroxy forms, respectively. 3 alpha-Hydroxyecdysteroid formation is only detected in the gut; all tissues contain the two other activities. It appears that conversion of 3 beta- to 3 alpha-hydroxyecdysteroids proceeds through 3-dehydro compounds. The biological significance of these reactions is discussed.</p>","PeriodicalId":13015,"journal":{"name":"Hoppe-Seyler's Zeitschrift fur physiologische Chemie","volume":"365 7","pages":"809-17"},"PeriodicalIF":0.0000,"publicationDate":"1984-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/bchm2.1984.365.2.809","citationCount":"47","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Hoppe-Seyler's Zeitschrift fur physiologische Chemie","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1515/bchm2.1984.365.2.809","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 47
Abstract
The formation and fate of 3-dehydroecdysteroids were studied in vitro in tissue extracts from Pieris brassicae (Lepidoptera). Three cytosolic enzymes are involved: an ecdysone oxidase converts 3 beta-hydroxyecdysteroids into 3-dehydroecdysteroids, and two different reductases, requiring NADPH as cofactor, transform 3-dehydroecdysteroids into the 3 alpha-hydroxy- or the 3 beta-hydroxy forms, respectively. 3 alpha-Hydroxyecdysteroid formation is only detected in the gut; all tissues contain the two other activities. It appears that conversion of 3 beta- to 3 alpha-hydroxyecdysteroids proceeds through 3-dehydro compounds. The biological significance of these reactions is discussed.
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