Electrophysiology of raccoon cuneocerebellar neurons.

J H Haring, M J Rowinski, B H Pubols
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引用次数: 20

Abstract

Electrophysiological experiments were undertaken in order to locate and functionally characterize cells of the raccoon main cuneate nucleus (MCN) that can be activated by electrical stimulation of the cerebellum. A total of 98 such units were studied in pentobarbital sodium-anesthetized, methoxyflurane-anesthetized, or decerebrate preparations. Aside from a greater likelihood of resting discharge in the decerebrate preparations, no appreciable variability in physiological properties of the neurons could be attributed to differences in the type of preparation. Using constant latency of response and ability to be blocked by collision as principal criteria, both antidromically (n = 31) and synaptically (n = 67) activated neurons of the main cuneate nucleus could be identified. A small number of MCN neurons could be activated by both cerebellar and thalamic stimulation, but no unit was antidromically activated from both locations. MCN neurons projecting to the cerebellum are located primarily in the ventral polymorphic cell region of the nucleus at and rostral to the obex, corresponding to the "medial tongue" region of Johnson et al. (1968). In contrast, neurons synaptically activated from the cerebellum are found throughout the dorsoventral extent of the rostral MCN, including the "clusters" region. The majority of antidromically activated units responded to mechanical stimulation of deeper tissues, and most of these were activated by muscle stretch. Only a small portion (13-15%) of either antidromically or synaptically activated units were classed as light touch units with peripheral receptive fields (RFs) restricted to glabrous surfaces of the forepaw. Glabrous skin RFs located on the digital surfaces are smaller than those located on the palm pads. In both cases, RFs are larger than those associated with primary afferent fibers, but toward the low end of the distribution for MCN neurons not activated by cerebellar stimulation. All MCN units activated by cerebellar stimulation, regardless of modality, respond to mechanical stimulation with trains of irregularly spaced single spikes. Glabrous skin cutaneous mechanoreceptive MCN neurons, whether rapidly or slowly adapting, respond to ramp indentations with an instantaneous frequency which may be described as a power function of ramp velocity, with exponents less than one. These values are in the same range as those previously reported for primary afferents of the cuneate fasciculus (Pubols and Pubols, 1973).

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浣熊大脑小脑神经元的电生理。
为了定位和功能表征可被小脑电刺激激活的浣熊主楔形核(MCN)细胞,进行了电生理实验。共有98个这样的单位在戊巴比妥钠麻醉、甲氧基氟醚麻醉或去脑制剂中进行了研究。除了在去脑制剂中更有可能静息放电外,神经元的生理特性没有明显的变化可以归因于制剂类型的差异。以恒定的反应潜伏期和被碰撞阻断的能力为主要标准,可以识别主楔形核的反方向(n = 31)和突触性(n = 67)激活的神经元。少量MCN神经元可以同时被小脑和丘脑刺激激活,但没有单元可以同时被两个位置激活。投射到小脑的MCN神经元主要位于核的腹侧多态细胞区和喙侧到臀部,与Johnson等人(1968)的“内侧舌”区域相对应。相反,由小脑突触激活的神经元遍布吻侧MCN的背腹侧,包括“簇”区域。大多数反生理激活单元对深层组织的机械刺激有反应,其中大多数是由肌肉拉伸激活的。只有一小部分(13-15%)的反性或突触激活单元被归类为轻触单元,外周接受野(RFs)仅限于前爪的无毛表面。位于数字表面的无毛皮肤rf比位于手掌垫上的rf要小。在这两种情况下,RFs都大于与初级传入纤维相关的RFs,但对于未被小脑刺激激活的MCN神经元,RFs分布的低端。所有被小脑刺激激活的MCN单元,无论其方式如何,都以不规则间隔的单尖峰序列响应机械刺激。无毛皮肤皮肤机械感受性MCN神经元,无论是快速还是缓慢适应,都以瞬时频率对斜坡凹痕作出反应,该频率可以描述为斜坡速度的幂函数,其指数小于1。这些值与先前报道的楔形束初级事件的值范围相同(Pubols and Pubols, 1973)。
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