{"title":"Stimulatory effect of polyprenoic acid E5166 on 2-deoxy-D-glucose uptake by Swiss 3T3 cells.","authors":"T Hasegawa, H Nishino","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>3,7,11,15-Tetramethyl-2,4,6,10,14-hexadecapentaenoic acid (E5166), a synthetic analog of retinoic acid, increased 2-deoxy-D-glucose (2DG) uptake in a dose and time-dependent manner in mouse fibroblasts, Swiss 3T3 cells. Kinetic analysis of 2DG uptake showed that the Vmax for 2DG uptake by E5166-treated cells was greater than that of the control, while the Km values were essentially the same. Most of the E5166-stimulated 2DG uptake was suppressed by cycloheximide, although part of the stimulation always remained despite of the cycloheximide treatment. These results suggest that E5166-induced stimulation of 2DG uptake is due to the increase in the synthesis and recycle of hexose transporters (carriers) in Swiss 3T3 cells.</p>","PeriodicalId":75427,"journal":{"name":"Acta vitaminologica et enzymologica","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"1984-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Acta vitaminologica et enzymologica","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
3,7,11,15-Tetramethyl-2,4,6,10,14-hexadecapentaenoic acid (E5166), a synthetic analog of retinoic acid, increased 2-deoxy-D-glucose (2DG) uptake in a dose and time-dependent manner in mouse fibroblasts, Swiss 3T3 cells. Kinetic analysis of 2DG uptake showed that the Vmax for 2DG uptake by E5166-treated cells was greater than that of the control, while the Km values were essentially the same. Most of the E5166-stimulated 2DG uptake was suppressed by cycloheximide, although part of the stimulation always remained despite of the cycloheximide treatment. These results suggest that E5166-induced stimulation of 2DG uptake is due to the increase in the synthesis and recycle of hexose transporters (carriers) in Swiss 3T3 cells.