{"title":"Adaptation of a protein a binding assay for measurement of immune complexes in sera of rats.","authors":"W M Siag, J M Jones","doi":"10.3109/08820138309066870","DOIUrl":null,"url":null,"abstract":"<p><p>We describe a simple and sensitive method for the detection of circulating immune complexes (CIC) in rats and have applied the method to test sera from tumor-bearing rats. CIC were precipitated preferentially with 6% polyethylene glycol, dissolved, and then bound to Staphylococcus aureus that are Protein A (PA) positive. Rabbit-anti-rat IgG (RARG) antibodies were then added and followed by 125I-PA. The RARG sandwich antibody enhanced the sensitivity of detection of CIC in rats at least 5 fold compared to that observed using 125I-PA without RARG.</p>","PeriodicalId":13417,"journal":{"name":"Immunological communications","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"1983-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.3109/08820138309066870","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Immunological communications","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.3109/08820138309066870","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
We describe a simple and sensitive method for the detection of circulating immune complexes (CIC) in rats and have applied the method to test sera from tumor-bearing rats. CIC were precipitated preferentially with 6% polyethylene glycol, dissolved, and then bound to Staphylococcus aureus that are Protein A (PA) positive. Rabbit-anti-rat IgG (RARG) antibodies were then added and followed by 125I-PA. The RARG sandwich antibody enhanced the sensitivity of detection of CIC in rats at least 5 fold compared to that observed using 125I-PA without RARG.
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