Lectin-resistant CHO cells: selection of new mutant phenotypes.

Abstract

Cytotoxic plant lectins select for mutants which exhibit unique structural changes in surface carbohydrates reflecting specific defects in glycosylation reactions. However, lectins are not highly specific selective agents and, as a result, only the most frequently occurring mutants are obtained from single lectin selections. We have previously shown that the specificity of lectin selections may be improved by utilizing a combination of lectins added together or sequentially. This strategy has now been further exploited in the search for novel lectin-resistant mutants of Chinese hamster ovary cells. Five new LecR phenotypes have been uncovered. One belongs to a new, recessive complementation group, two behave dominantly in somatic cell hybrids, and the remaining two appear to represent new phenotypes which fall into previously described complementation groups.