[Immuno and enzyme histochemical demonstration of granulocytes--assay of a morphometric evaluation].

Abstract

Using the naphthol AS-D-chloroacetate esterase method and the indirect immunoperoxidase technique we tried to stain selectively granulocytes in the lung and in the liver. For the immuno technique we used an antibody raised in rabbits against dog granulocytes, which were prepared by a new separation technique. Comparing the two staining methods we found the immunoperoxidase to be superior at least for quantitative evaluation. In a pilot study with polytraumatized dogs versus controls we could detect a more pronounced leucostasis within the trauma group. This was similar to a parallel study which used radioactive labeled granulocytes where a significant increase could be shown. Granulocytes, at least in part, seem to be important for cellular changes within the "lung in shock".