[Formation of stable diploid bacteria by fusion of protoplasts of Bacillus subtilis and the effect of rec- mutations on the fusion products formed].

C Lévi-Meyrueis, C Sanchez-Rivas, P Schaeffer
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Abstract

A minority among the prototrophic clones formed when protoplasts from two polyauxotrophic strains of B. subtilis are fused has been shown to be diploid, carrying the parental deficient alleles in their DNA, and generally segregating auxotrophs when grown in nutrient medium. This is true whether the strains being fused are Rec+ or Rec-, since the rec- mutations used hardly affect recombinations occurring between chromosomes in diploid Bacteria.

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[枯草芽孢杆菌原生质体融合形成稳定的二倍体细菌及rec突变对融合产物的影响]。
当两个多营养不良菌株的原生质体融合形成的原生营养无性系中有少数显示为二倍体,在其DNA中携带亲本缺陷等位基因,并且在营养培养基中生长时通常会分离营养不良。无论融合的菌株是Rec+还是Rec-,这都是正确的,因为在二倍体细菌中,Rec-突变几乎不影响染色体之间发生的重组。
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