[Micromethod of measuring the 3 brain isoenzymes of enolase].

Abstract

The brain of the vertebrates contains three isozymic forms of the glycolytic enzyme enolase, two of which are neuron specific. These proteins could therefore be utilized as biochemical markers for various studies of neurons. We have devised a separation procedure which allows the measurement of the three enolase forms from minute amounts of tissue (50 to 100 microgram total protein). We show that this method can be applied to studies of brain microstructures, in vivo and in vitro (clonal cell lines) neural differentiation, and in measurements in biological fluids.