Su-Chen Li, Makio Asakawa, Yoshio Hirabayashi, Yu-Teh Li
{"title":"Isolation of two endo-β-N-acetylglucosaminidases from fig latex","authors":"Su-Chen Li, Makio Asakawa, Yoshio Hirabayashi, Yu-Teh Li","doi":"10.1016/0005-2744(81)90171-6","DOIUrl":null,"url":null,"abstract":"<div><p>Two endo-<em>β</em>-<em>N</em>-acetylglucosaminidases (mannosyl-glycoprotein <span><math><mtext>1,4-N-</mtext><mtext>acetamidodeoxy</mtext><mtext>-β-</mtext><mtext>d</mtext><mtext>-</mtext><mtext>glycohydrolase</mtext></math></span>, EC 3.2.1.96) (type F-I and type F-II) have been isolated from fig latex. At pH 7.0, type F-1 was retained by the DEAE-Sephadex A-50 column, whereas type F-II was not adsorbed by the column. The optimum pH of type F-I was found to be pH 5.9 and type F-II, pH 5.4. Type F-I enzyme hydrolyzes the tri-mannosyl derivatives di-<em>N</em>-acetylglucosaminylasparagine faster than the penta- or hexa-mannosyl compounds. Type F-II hydrolyzes the penta- and hexa-mannosyl derivatives, but not the tri-mannosyl compound.</p></div>","PeriodicalId":100159,"journal":{"name":"Biochimica et Biophysica Acta (BBA) - Enzymology","volume":"660 2","pages":"Pages 278-283"},"PeriodicalIF":0.0000,"publicationDate":"1981-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0005-2744(81)90171-6","citationCount":"11","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biochimica et Biophysica Acta (BBA) - Enzymology","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/0005274481901716","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 11
Abstract
Two endo-β-N-acetylglucosaminidases (mannosyl-glycoprotein , EC 3.2.1.96) (type F-I and type F-II) have been isolated from fig latex. At pH 7.0, type F-1 was retained by the DEAE-Sephadex A-50 column, whereas type F-II was not adsorbed by the column. The optimum pH of type F-I was found to be pH 5.9 and type F-II, pH 5.4. Type F-I enzyme hydrolyzes the tri-mannosyl derivatives di-N-acetylglucosaminylasparagine faster than the penta- or hexa-mannosyl compounds. Type F-II hydrolyzes the penta- and hexa-mannosyl derivatives, but not the tri-mannosyl compound.
从无花果乳胶中分离到两个内切-β- n -乙酰氨基葡萄糖氨基酶(甘露糖糖蛋白1,4- n -乙酰氨基脱氧-β-d-糖水解酶,EC 3.2.1.96) (F-I型和F-II型)。在pH 7.0时,型F-1被DEAE-Sephadex A-50柱保留,而型F-II不被柱吸附。F-I型的最佳pH为5.9,F-II型的最佳pH为5.4。F-I型酶水解三甘露糖基衍生物二n -乙酰氨基葡萄糖天冬氨酸的速度比五甘露糖基或六甘露糖基化合物快。F-II型水解五甘露糖基和六甘露糖基衍生物,但不能水解三甘露糖基化合物。