{"title":"Purification and characterization of a mouse serum protein with growth inhibitory properties in vitro against tumor cell lines.","authors":"S Gundersen, E Paus, S Funderud","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>A factor which is responsible for the growth inhibitory properties of certain mouse sera and related to NK-activity, has been studied. The factor was isolated from hybrid B6D2F1 (C57Bl/6 x DBA/2) serum, which is histo-compatible with the mouse tumour (B16 melanoma) used and has high NK-activity. Growth inhibitory activity was measured in an in vitro assay. It was independent of complement activation. The responsive factor was isolated and characterized by ion exchange chromatography. Concanavalin A affinity chromatography, gel filtration and iso-electric focusing. It appears to be a protein and had been labelled growth-inhibitory factor (GIF). It has a molecular weight of 230 000-260 000 daltons and an iso-electric point in the pH range 4.6-5.0. It was not retained on Concanavalin A columns.</p>","PeriodicalId":77653,"journal":{"name":"Acta pathologica, microbiologica, et immunologica Scandinavica. Section C, Immunology","volume":"91 2","pages":"129-35"},"PeriodicalIF":0.0000,"publicationDate":"1983-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Acta pathologica, microbiologica, et immunologica Scandinavica. Section C, Immunology","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
A factor which is responsible for the growth inhibitory properties of certain mouse sera and related to NK-activity, has been studied. The factor was isolated from hybrid B6D2F1 (C57Bl/6 x DBA/2) serum, which is histo-compatible with the mouse tumour (B16 melanoma) used and has high NK-activity. Growth inhibitory activity was measured in an in vitro assay. It was independent of complement activation. The responsive factor was isolated and characterized by ion exchange chromatography. Concanavalin A affinity chromatography, gel filtration and iso-electric focusing. It appears to be a protein and had been labelled growth-inhibitory factor (GIF). It has a molecular weight of 230 000-260 000 daltons and an iso-electric point in the pH range 4.6-5.0. It was not retained on Concanavalin A columns.
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