{"title":"Estimation of Vibrio cholerae and Escherichia coli heat-labile enterotoxin by enzyme-linked immunosorbent assay (ELISA).","authors":"I Kétyi, A S Pácsa","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>By Enzyme-Linked Immunosorbent Assay (ELISA) purified cholera and Escherichia coli enterotoxins can be detected as sensitively as by CHO cells. There is a linear relationship between toxin concentrations and extinction values. In plates sensitized with toxins, antitoxins can be titrated with high sensitivity. ELISA cross-titration experiments demonstrate the existing antigenic relationship between cholera toxin and heat labile E. coli enterotoxin. Plates sensitized with either anti-E, coli-IgG or anti-cholera-IgG are suitable for detecting both cholera toxin, and E. coli LT. ELISA seems to be a simple, sensitive and economic method for quantitation of enterotoxins and toxin-specific antibodies.</p>","PeriodicalId":75387,"journal":{"name":"Acta microbiologica Academiae Scientiarum Hungaricae","volume":"27 1","pages":"89-97"},"PeriodicalIF":0.0000,"publicationDate":"1980-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Acta microbiologica Academiae Scientiarum Hungaricae","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
By Enzyme-Linked Immunosorbent Assay (ELISA) purified cholera and Escherichia coli enterotoxins can be detected as sensitively as by CHO cells. There is a linear relationship between toxin concentrations and extinction values. In plates sensitized with toxins, antitoxins can be titrated with high sensitivity. ELISA cross-titration experiments demonstrate the existing antigenic relationship between cholera toxin and heat labile E. coli enterotoxin. Plates sensitized with either anti-E, coli-IgG or anti-cholera-IgG are suitable for detecting both cholera toxin, and E. coli LT. ELISA seems to be a simple, sensitive and economic method for quantitation of enterotoxins and toxin-specific antibodies.
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